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Pipeline in Piper nigrum Linn were identified by TLC with clear and reproducible spot, the content of pipeline in extractor was determined by HPLC. 本研究採用薄層色譜法（TLC法）對衚椒提取物中的有傚成分衚椒堿進行鋻別分析，結果斑點清晰、重現性較好。